Skip to main content
. 2013 Aug;183(2):402–412. doi: 10.1016/j.ajpath.2013.05.001

Figure 2.

Figure 2

Enhanced immune responses in IL-10−/− mice with GN. A: Splenocytes were prepared from WT or IL-10−/− mice with GN induced by aGBM, and the cells were activated with SG for 24 hours. IL-17–producing CD4+ T cells were analyzed by FACS, and IL-17 protein levels in the supernatants were determined by ELISA. ∗P < 0.05, versus immunized IL-10−/− mice. B: WT or IL-10−/− mice were sensitized with SG for 5 days, and the mice were then injected i.p. with sheep anti-mouse GBM antibody at a dose of 30 mg per mouse. Renal injuries were assessed 14 days later. The infiltration of immune cells was analyzed by immunostaining in the kidneys. Quantification of T cells (C), macrophages (D), and neutrophils (E) in the kidneys of WT and IL-10−/− mice. gcs, glomerular cross section. Data are given as means ± SD.