Skip to main content
. 2013 Aug;183(2):402–412. doi: 10.1016/j.ajpath.2013.05.001

Figure 5.

Figure 5

Transfer of IL-10−/− spleen cells induces more severe GN in Rag1−/− mice. A: Spleen cells from WT and IL-10−/− mice were prepared and were injected i.p. into Rag1−/− mice. Beginning the next day, mice were sensitized with SG for 5 days, and the mice were then injected i.p. with sheep anti-mouse GBM globulin at a dose of 30 mg per mouse. Renal injuries were assessed 20 days later. A: PAS staining of glomeruli in mice reconstituted with either WT or IL-10−/− spleen cells. B: Quantification of histologic injury showed aggravation of GN in mice reconstituted with either WT or IL-10−/− spleen cells. gcs, glomerular cross section. ∗P < 0.05 versus mice transferred with IL-10−/− spleen cells. C: Spleens were enlarged and spleen cells were significantly increased in Rag1−/− mice reconstituted with IL-10−/− splenocytes. D: Splenocytes were prepared from Rag1−/− mice reconstituted with either WT or IL-10−/− spleen cells with GN induced by aGBM globulin, and the cells were activated with SG for 24 hours. The supernatants were harvested, and IL-17 protein levels were determined by ELISA. Data are given as means ± SD.