Figure 1.

Porcine liver decellularization. Whole native liver (A) before perfusion with Triton X-100 (B) and SDS (C). Indicated by panels on the SDS-treated liver, the preserved major (D) and minor (E) vessels are visible at the end of the protocol. Native liver sections stained with H&E (F) and Masson’s trichrome (G) help illustrate the complete cellular removal seen in their respective decellularized counterparts (H and I). Arrows indicate preserved vessels in decellularized matrices compared with native structures. Scale bar = 100 μm (F–I).