Table 2.

	MCF-10F	E2	C5	T4	p value
Invasiveness	162 ± 11	683 ± 48	1322 ± 145	503 ± 231	≤0.001a
Ductulogenesis in collagen matrix	31.8 ± 1.4	5.1 ± 0.7	0.7 ± 0.4	0.7 ± 0.2	≤0.001b
Mass formation in collagen
<250 nm	–	334 ± 9.4	12 ± 2	41.7 ± 4	≤0.001c
>250 nm	–	24 ± 8.6	1 ± 0.4	13.1 ± 1.9	0.044d
Colony forming in agar
<250 μm	191.5 ± 8.5	619.2 ± 53.7	281.7 ± 29	113 ± 20	0.01e
>250 μm	–	–	175 ± 20	91 ± 6.6	0.008f

ANOVA followed by Holm–Sidak test was used for the comparisons of above-mentioned values except colony formation (bottom line) and results were expressed as ±SEM (standard error of the mean)

^aC5 cells showed higher invasion rates than the others

^bMCF-10F cells showed higher rate of ductal formation in collagen matrix when compared to the other groups. The comparison between E2 and C5 was also significant. No mass formation was observed in cultured MCF-10F cells

^cThe highest values observed in E2 cells. The all comparisons were significant

^dE2 values were higher than C5. Other comparisons were insignificant

^eMean colony numbers in E2 group were significantly higher than other groups. In addition, the comparison between C5 and T4 groups was statistically significant. However, other comparisons were insignificant (ANOVA followed by Holm–Sidak test). Other group comparisons in the aspect of colony formation in agar-methocel were insignificant

^fColony formation bigger than 250 μm was observed in C5 and T4 groups. Mean colony number in C5 group was statistically higher than T4 (Student t test)