Inhibition of NSEP1 protein expression by siRNAs. Western blot assay for detection of NSEP1 (top part) and β-actin (bottom part) from lysates of HEK 293 cells transfected with siRNA duplexes as indicated in the bottom margin: none, no siRNA; GFP, GFP-silencing siRNA duplex; 1, NSEP1 siRNA duplex 1; 2, NSEP1 siRNA duplex 2; 3, NSEP1 siRNA duplex 3. HEK 293 cells were transfected with the indicated siRNA constructs, incubated 48 h, then lysed and analyzed by Western blotting for the proteins indicated in the left margin.