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. Author manuscript; available in PMC: 2014 Jun 27.
Published in final edited form as: Immunity. 2013 Jun 27;38(6):1142–1153. doi: 10.1016/j.immuni.2013.05.016

Figure 6. Incubation in low-K+ medium is sufficient to activate NLRP3.

Figure 6

(A) LPS-primed WT and Nlrp3−/− BMDMs were incubated in K+-free medium and the release of IL-1β (bars) and intracellular content of K+ (solid squares) were measured at the specified time points. (B) LPS-primed WT and Nlrp3−/− BMDMs were incubated for 2 hrs in medium containing the specified [K+] and the release of IL-1β (bars) and intracellular content of K+ (solid squares) were measured. (C) LPS-primed WT and Nlrp3−/− BMDMs were treated with ouabain for 2 hours in IMDM or K+-free medium and the release of IL-1β and the intracellular content of K+ were determined. IL-1β secretion was analyzed by ELISA. K+ determinations were performed in Nlrp3−/− cells. Values represent mean ± standard deviation (n=3). *, statistically significant (p < 0.05, WT vs. Nlrp3−/−). Results are representative of at least three separate experiments. See also Figure S4.