. Author manuscript; available in PMC: 2013 Aug 1.

Published in final edited form as: Acta Biomater. 2012 Apr 3;8(7):2815–2823. doi: 10.1016/j.actbio.2012.03.042

Table 3.

Total cellular protein in the primary synovium was compared to the revision pseudomembranes using Western blotting staining for CD68, the M1 marker HLA-DR, and the M2 marker, CD163.

	Primary Synovium	Revision Pseudomembrane
M1/CD68	0.25 ± 0.03	18.31 ± 11.59
M2/CD68	1.20 ± 0.35	1.07 ± 0.35
M1/M2	0.24 ± 0.04	12.95 ± 7.51

M1 and M2 ratios in the primary synovium and revision pseudomembrane assessed by Western blotting using 50 µg of protein per well. The M1/CD68 ratio in the primary synovium was 0.25 ± 0.03, compared to 18.31 ± 11.59 in the revision pseudomembrane. The M2/CD68 ratio in the synovium was 1.20 ± 0.35, compared to 1.07 ± 0.35 in the revision pseudomembrane. The M1/M2 ratio in the synovium was 0.24 ± 0.04, compared to 12.95 ± 7.51 in the revision pseudomembrane, suggesting that there is a predominance of M1 macrophages in the revision pseudomembrane.