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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Immunol. 2013 Jul 1;191(3):1393–1403. doi: 10.4049/jimmunol.1300052

Figure 1. Hsp70 is inducted and secreted by MLEC during hyperoxia.

Figure 1

A) Hsp70 protein in WT and TLR4−/− MLEC by western blot after a time course of hyperoxia. β-tubulin as loading control. B) Hsp70 protein in WT MLEC supernatant was measured by ELISA after 8h, 16h, and 24h of hyperoxia. RA, room air control. * p <0.05 vs RA. C) WT and TLR4−/− MLEC were treated with recombinant human Hsp70 protein at 8.65µg/ml and were exposed to 72h of hyperoxia. RA, room air control. Graphical quantitation of flow cytometry analysis of apoptosis was determined. The values are expressed as mean ± SD. *p<0.05, vs RA WT; #p<0.05 vs hyperoxia WT (experiments were performed in triplicates).