Figure 3.

Response of toe1⁺ to chlorpromazine treatment. (A) Loss of toe1⁺ results in sensitivity to chlorpromazine. Exponentially growing toe1Δ and wild-type strains were spot diluted on YES medium lacking or containing chlorpromazine (100 µg/ml) and incubated for 3 days at 30°. (B) Transcriptome profiling of toe1Δ and wild-type strains treated with chlorpromazine. toe1 putative target genes were induced in the wild type but not in the toe1Δ strain upon chlorpromazine treatment (left and middle columns, respectively). As a result, the expression of the putative target genes is lower in the toe1Δ strain relative to wild type when treated with chlorpromazine (right column). The microarray experiments were performed with dye reversal. Relative expression ratios are indicated in the color bar. Chloropromazine treatment for the transcriptome profiling experiments was 300 µg/ml for 1.5 hr.