Figure 7.

che-6 codes for a cyclic nucleotide-gated ion channel. (A) che-6 gene structure and alleles. The alanine-encoding codon mutated in e1126 to a threonine-encoding codon resides in the nucleotide-binding domain. Generally, within nucleotide-binding domains, this position is either an alanine or a glycine (Kaupp and Seifert 2002). (B) Schematic protein structure of CHE-6s. (C) Phylogenetic tree built at the www.phylogeny.fr suite (Dereeper et al. 2008). Full-length protein sequence was used an Hyperpolarization-activated cyclic nucleotide-gated channel (HCN; no worm homologs) were used to root the tree. A similar clustering is observed if only the cNMP domain or the ion channel domain is used (Figure S2). (D) che-6^prom::gfp expression pattern. The left panels show the overview of expression in several adult head neurons. The two smaller panels on the right show an image of the head region of a transgenic animal coexpressing che-6^prom::gfp (hanEx24) and ASE + AWC-expressed ceh-36::mCherry (otIs264), revealing overlap of expression in ASE and AWC.