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Isogenic, donorless wild-type (wt) and exo1Δsgs1Δ strains were grown asynchronously, and analyzed by ChIP in the region surrounding the DSB for (a) recruitment of the indicated chromatin modifying enzyme subunits 4 hours after induction (dotted line indicates the HO cut site), and (b) levels of γH2AX at the indicated time points after induction. Data shown represent at least two biological replicates; error bars represent s.e.m.
