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. Author manuscript; available in PMC: 2013 Dec 28.
Published in final edited form as: Nat Commun. 2013;4:2084. doi: 10.1038/ncomms3084

Figure 5. Rad51 is necessary for recruitment of chromatin regulators.

Figure 5

Isogenic, donorless wild-type (wt), and (a) rad51Δ or (b) rad54Δ strains were arrested in G2 with nocodazole, and analyzed by ChIP for recruitment of the indicated chromatin modifying enzyme subunits to the region the DSB region at the indicated time points after DSB induction. A dotted line indicates the HO cut site. (c,d) Levels of γH2AX determined by ChIP in the DSB region of experiments described in panels (a) and (b), repsectively. Data shown represent at least two biological replicates; error bars represent s.e.m.