Table 2.

Thrombin generation assay parameter values

	Latent Time (min)	Peak Time (min)	Peak Value (nM)	ETP (nM·min)
WT	11.9 ± 0.2 (1.00)	18.6 ± 0.1 (1.00)	108.9 ± 9.1 (1.00)	957.9 ± 41.1 (1.00)
G1750C/R2116C	11.5 ± 1.2 (0.97)	20.6 ± 1.0 (1.11)	45.9 ± 4.5 (0.42)	514.2 ± 19.7 (0.54)
A1866C/S2119C	13.2 ± 3.5 (1.11)	25.1 ± 2.7 (1.35)	33.4 ± 1.5 (0.31)	408.8 ± 36.5 (0.43)
R2116A	10.4 ± 0.2 (0.87)	18.2 ± 0.1 (0.98)	76.5 ± 8.6 (0.70)	744.1 ± 91.3 (0.78)
R2116V	12.6 ± 0.2 (1.06)	18.6 ± 0.1 (1.00)	69.4 ± 3.5 (0.64)	595.4 ± 4.4 (0.62)
S2029C/P2292C	7.8 ± 0.7 (0.66)	15.1 ± 0.3 (0.81)	139.6 ±12.8 (1.28)	1076.0 ± 24.0 (1.12)

Thrombin generation assays in the presence of 0.25 nM factor VIII proteins, 0.5 pM rTF, and 4 μM PSPCPE vesicles were performed and parameter values were calculated as described in Methods. Data represents the average values ± SD of triplicate samples. Values in parentheses are relative to the WT value. The single letter code is used to designate amino acid residues, G (Gly), R (Arg), A (Ala), S (Ser), C (Cys), V (Val), and P (Pro).