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Real-time PCR analysis of the Cx mRNA expression in OSCC cells after ATRA treatment. The gene expression levels in SCC9 and Tca8113 cells were analyzed separately and were calculated in terms of fold change by using the 2-ΔΔCt equation. Colums represent the mean of three determinations for each treatment condition. Error bars represent the range factor difference (2-ΔΔCt-ΔCt SD and 2ΔΔCt+ΔCt SD). Statistical significance was calculated by Student t test; *P< 0.05 relative to control.
