Figure 2.

Asparaginase activity is measured spectrophotometrically using a calibration curve. A: Absorption readings are taken every 20 seconds for 15 minutes after initiating the reaction to capture changes in absorbance over time associated with asparaginase activity. Only the linear portions of the curve are used to estimate the reaction rate (ΔmOD/minute). B: A calibration curve is constructed by plotting the known asparaginase concentrations of the calibrators (0.025-2.2 IU/mL) versus the truncated reaction rates. The regression equation is used to determine the asparaginase activity of a sample with unknown activity. The coefficient of determination (R²) for the regression line was estimated to be 0.998.