Fig. 4.

Effects of macrophage depletion and TNF-$\alpha$ knockout on SHG and IF signals in the E0771 tumor. (a) SHG-producing fibers in E0771 tumors in wild-type/PBSL mice were significantly brighter compared to all other groups ($p<0.05$ for all comparisons). In addition, SHG-producing fibers in E0771 tumors in wild-type/ClodL mice were significantly dimmer compared to TNF-${\alpha }^{(-/-)}/\mathrm{PBSL}$ tumors ($p<0.05$). No other group was found significantly different from another ($p>0.05$). $n=21\mathrm{wt}/\mathrm{PBSL}$, $16\mathrm{wt}/\mathrm{ClodL}$, $n=15$TNF-${\alpha }^{(-/-)}/\mathrm{PBSL}$, and $n=15$ TNF-${\alpha }^{(-/-)}/\mathrm{ClodL}$). (b) Collagen I immunofluorescence (IF) was significantly lower in E0771 tumors in wild-type/PBSL mice compared to all other groups ($p<0.05$ for all comparisons). In addition, collagen I IF in E0771 tumors in TNF-${\alpha }^{(-/-)}/\mathrm{PBSL}$ mice was significantly lower than in E0771 tumors in TNF-${\alpha }^{(-/-)}/\mathrm{ClodL}$ mice. No other group was found significantly different from another ($p>0.05$).