Figure 2. Validation of expression of 8 miRNAs using qRT-PCR.
A) The expression of 8 miRNAs (miR-146b-5p, miR-21, miR-375, miR-148a, miR-31, miR-133b, miR-451, and miR-200a) was evaluated by qRT-PCR using 33 gastric adenocarcinoma and 47 normal gastric tissues. miR-191 and miR-103a-5p were used for normalization and the data are shown as relative expression level. The horizontal bars indicate the arithmetic mean. Statistical analysis was performed using Student's t-test and a p ≤0.05 was considered statistically significant. B) Heat map analysis of the relative expression level of the eight miRNAs in gastric adenocarcinoma and normal gastric tissues.