Table 4. Activity of S. tropicalis peptides against a broad range of cell types.
Gram-negative bacteria | Gram-positive bacteria | Eukaryote microorganisms | vertebrate cells | |||||
E. coli | P. aeruginosa | S. aureus | M. luteus | S. cerevisiae | T. brucei | Erythrocytes | T-lymphocytes | |
Peptide | MIC (µM) | MIC (µM) | MIC (µM) | MIC (µM) | MIC (µM) | LC95 (µM) | HC50 (µM) | IC50 (µM) |
CPF-St4 | 2–4 | 4–8 | 1 | 0,5 | 16 | 2–4 | 64 | 2 |
CPF-St5 | 1 | 4–8 | 1 | 0,5 | 16 | 1–2 | 64 | 8 |
CPF-St6 | 16 | >256 | >256 | 4 | 64 | 16–256 | 256 | 32–64 |
CPF-St7 | 128 | >256 | 32 | 2 | 64 | 2–64 | 256 | 128 |
magainin-St1 | 64 | >256 | >256 | 32 | 128 | 8–>256 | >256 | >256 |
XPF-St4 | 32 | 128 | >256 | 0,5 | 64 | 4 | 256 | 64 |
XPF-St5 | 1–2 | 8–16 | 16 | 1 | 32 | 2–4 | 128 | 8–16 |
XPF-St1 | 4 | 32 | 32–64 | 0,5 | 32 | 2–64 | 128 | 16 |
XPF-St6 | 2–4 | 16 | 8–16 | 0,5 | 8 | 2–4 | 64 | 8 |
XPF-St7 | 8 | 32 | 128 | 1 | 32 | 2–64 | >256 | 16 |
XPF-St8 | 1 | 4 | 128 | 0,5 | 8 | 1–8 | 64 | 16 |
PFQa-St2 | 32 | 256 | 16 | 4 | 128 | 16–32 | 256 | 128 |
PGLa-St2 | 16 | 128 | >256 | 2 | 64 | 16–32 | >256 | 64 |
MIC, minimum inhibitory concentration, the lowest peptide concentration (in µM) in a series of twofold dilutions at which no growth was detected; LC95, the lowest peptide concentration at which at east 95% of parasites was killed after 30 minutes; HC50, the lowest peptide concentration causing at least 50% hemolysis; IC50, the lowest peptide concentration causing at least 50% inhibition of Concanavalin A-induced T-cell proliferation. In case of variation among repetitive tests, observed minima and maxima for these values are given, respectively.