Figure 7. Specificity of the viperin effects on modulation of lipid metabolism in HCMV infection.

(A–C) HFtelo cells stably expressing the indicated shRNAs were infected with recombinant HCMV.mVIP, in which the loci US7–US16 were replaced by doxycycline (Dox)-inducible mouse viperin-GFP, at an moi of 2 for 1 or 3 days. Dox (2 µg/ml) was added before infection. mRNA levels of GLUT4 and ChREBPs (A) and lipogenic enzymes (B) were measured. Data are presented as means ± SEM of triplicate samples and are representative of two individual experiments. Cells were stained with antibody specific to ADRP (C). The HCMV.mVIP-infected cells were detected by expression of viperin-GFP. The arrows indicate the accumulated LDs. Scale bar, 20 µm. (D) HFtelo cells expressing the indicated shRNAs were infected with HCMV.mVIP at an moi of 0.2, and supernatants and cells were harvested at 6 dpi. Dox (2 µg/ml) was added on day 0 and 3. Virus yield was quantified by a fluorescence-based virus infectivity assay. Data are presented as means ± SEM of duplicate samples and are representative of two individual experiments.