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. 2013 Aug 1;9(8):e1003529. doi: 10.1371/journal.ppat.1003529

Figure 1. MVs bind to S. pyogenes.

Figure 1

A) Pro-coagulant MVs were gold-labeled with annexin V (5 nm) and incubated with S. pyogenes bacteria in the presence of 1% plasma. Samples were processed by negative staining and analyzed in a transmission electron microscope at two different magnifications. Arrows point to annexin V-positive MVs and scale bars represent 500 nm (left) or 100 nm (right). B) An overview of an S. pyogenes bacterium opsonized with ctrl. (upper panel left site) and pro-coagulant MVs (upper panel right site) is shown (Scale bar 500 nm). Ctrl. MVs (left side) and pro-coagulant MVs (right side) were immunostained with a gold-labeled antibody against CD14 (middle panel) or tissue factor (lower panel) and incubated with S. pyogenes. The scale bar indicates 100 nm.