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. 2013 Aug 1;8(8):e71017. doi: 10.1371/journal.pone.0071017

Figure 1. AAV-mediated expression of myostatin pro-peptide D76A mutant attenuates aortic atheromatous lesion accumulation in Ldlr null mice.

Figure 1

The animals were injected with either AAV-D76A (D76A) or vehicle (veh) at 8 weeks of age, placed on a high fat diet seven weeks later, and euthanized after another 12 weeks. The entire aorta along with the aortic root was dissected for analysis. (A) En face staining with Sudan IV for lipid-rich lesions (stained red). The lower panel shows the quantitative lesion scores (mean ± SEM, n = 10). (B) Sections of the aortic root near the sinus stained with hematoxylin and eosin (H&E) for lesions built up along the vessel wall (LC: lipid core, VW: vessel wall). The lower panel shows the lesion area expressed as a percent of aortic area (mean±SEM, n = 10).