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. 2013 Aug 1;8(8):e70484. doi: 10.1371/journal.pone.0070484

Table 2. Primers used for PCR and DNA sequencing.

Primer name Sequence (5'–3') Gene/Plasmid
M13F TTTTCCCAGTCACGACG pGEM-T Easy Vector
M13R ACACAGGAAACAGCTATGACC pGEM-T Easy Vector
lcnI-R GCCATGTATTGGCCGTTT lcnI (Leucocin B-KM432Bz immunity protein)
lcnB-F AACATGAAATCTGCGGATAA lcnB (Leucocin B-KM432Bz precursor)
lcnB-R ACCAGAAACCATTTCCACCA lcnB (Leucocin B-KM432Bz precursor)
lcnBC-F TTGCATACCCTGTCTCCA lcnB-lcnC
tpase-F2 GGTAAAAGACGTGGTGATGT tra8
tpase-R1 GACCACGTAGATGACGTT tra8
lcnC-F1 CAGGCCGACATGTCCTA lcnC
lcnC-F2 GCGTGTTGCGTACTTC lcnC
lcnC-R GAAGTACGCAACACGC lcnC
lcnCD-R CCTTCCATGTCAGTTTTGGC Intergenic region lcnC-lcnD
lcnD-F1 TTTACGGACGCTAATGCC lcnD (ABC transporter)
lcnD-F2 TGAATAATGACCAAATGCAA lcnD (ABC transporter)
lcnD-R CTATTCAGCGCTTTAATCGT lcnD (ABC transporter)
lcnE-R1 AAGGAAAATTGCGGTAACG lcnE (accessory factor)
lcnE-F1 CGTTACCGCAATTTTCCAA lcnE (accessory factor)
lcnE-R2 CATAGCACTTTGGCTGGAA lcnE (accessory factor)
lcnE-R3 TAGCCTGAATCGTCGC lcnE (accessory factor)
27F CGGTGTGTCAAGGCCC 16S ribosomal RNA
1385R GAGTTTGATCCTGGCTCAG 16S ribosomal RNA