Table 2. Primers used for PCR and DNA sequencing.
Primer name | Sequence (5'–3') | Gene/Plasmid |
M13F | TTTTCCCAGTCACGACG | pGEM-T Easy Vector |
M13R | ACACAGGAAACAGCTATGACC | pGEM-T Easy Vector |
lcnI-R | GCCATGTATTGGCCGTTT | lcnI (Leucocin B-KM432Bz immunity protein) |
lcnB-F | AACATGAAATCTGCGGATAA | lcnB (Leucocin B-KM432Bz precursor) |
lcnB-R | ACCAGAAACCATTTCCACCA | lcnB (Leucocin B-KM432Bz precursor) |
lcnBC-F | TTGCATACCCTGTCTCCA | lcnB-lcnC |
tpase-F2 | GGTAAAAGACGTGGTGATGT | tra8 |
tpase-R1 | GACCACGTAGATGACGTT | tra8 |
lcnC-F1 | CAGGCCGACATGTCCTA | lcnC |
lcnC-F2 | GCGTGTTGCGTACTTC | lcnC |
lcnC-R | GAAGTACGCAACACGC | lcnC |
lcnCD-R | CCTTCCATGTCAGTTTTGGC | Intergenic region lcnC-lcnD |
lcnD-F1 | TTTACGGACGCTAATGCC | lcnD (ABC transporter) |
lcnD-F2 | TGAATAATGACCAAATGCAA | lcnD (ABC transporter) |
lcnD-R | CTATTCAGCGCTTTAATCGT | lcnD (ABC transporter) |
lcnE-R1 | AAGGAAAATTGCGGTAACG | lcnE (accessory factor) |
lcnE-F1 | CGTTACCGCAATTTTCCAA | lcnE (accessory factor) |
lcnE-R2 | CATAGCACTTTGGCTGGAA | lcnE (accessory factor) |
lcnE-R3 | TAGCCTGAATCGTCGC | lcnE (accessory factor) |
27F | CGGTGTGTCAAGGCCC | 16S ribosomal RNA |
1385R | GAGTTTGATCCTGGCTCAG | 16S ribosomal RNA |