Figure 7. Identification of FtsZ and MinD from co-IP performed with MinC during mid-exponential cultures of NCTC11637.

Proteins were eluted after co-IP experiments, samples were separated by SDS-PAGE and detected by Western blotting. Western blots were probed with (A) anti-MinC, (B) anti-FtsZ, and (C) anti-MinD. Lanes L, loading control consisting of whole-cell extract; lanes 1, proteins precipitated with anti-MinC_Hp; lanes 2, proteins precipitated with anti-FtsZ; lanes 3, proteins precipitated with anti-MinD.