Figure 9. The effects of MinC_Hp or MinC_Ec on cell viability and morphology of E. coli.

(A) E. coli MG1655 harboring the plasmid pBAD33, pCPY009 and pCPY010, respectively, were serially diluted 10-fold and spotted on LB plates supplemented with the indicated concentration of arabinose at 37°C. (B) DIC micrographs of cells grown in LB with indicated concentration of arabinose at 30°C. Scale bars, 10 µm. (C) SDS-PAGE (left panel) and Western blot (right panel) showing the levels of MinC_Hp in MG1655(pCPY009). Cells were grown with 0.002% (lanes 2 and 6), 0.02% (lanes 3 and 7), and 0.2% (lanes 4 and 8) of arabinose, respectively, at 30°C to an OD₆₀₀ of ca. 1.2 for subsequent immunoblot analysis. The MG1655(pCPY009) strain grown without arabinose induction was used as a control (lanes 1 and 5). (D) Selection of cells (I and II) were observed by fluorescence microscopy. IF microscopy to examine the localization of MinC_Hp in MG1655(pCPY009). Scale bars, 1 µm.