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. 2013 Aug 1;8(8):e71208. doi: 10.1371/journal.pone.0071208

Table 1. Strains and plasmids used in this study.

Strain or plasmid Genotype or description Source
Strains
E.coli
Top10 F-mcrΔ (mrr-hsdRMS-mcrBC) 80lacZΔM15 lacXΔ74 recA1 deoR araD139Δ (ara-leu) 7697 galU galKrpsL (StrR) endA1 nupG Invitrogen
BL21(DE3) F dcm ompT hsdS (rB mB ) gal λ(DE3) Stratagene
MG1655 wild-type [30]
H. pylori
NCTC 11637 wild-type, containing the entire minC ATCC 43504
PY1 11637, minC::cat This study
PY2 PY1, hp0405::PflaA-minCHp kan This study
PY2-5 PY1, hp0405::PflaA-minCEc kan This study
PY3 11637, hp0405::PflaA-minCHp kan This study
PY3-1 11637, hp0405::PflaA-minCEc kan This study
Plasmids
pAV35 chloramphenicol acetyltransferase (cat) cassette; Cmr [18]
pJMK30 kanamycin resistance (kan) cassette; Kanr [18]
pUC18 Cloning vector, Apr Fermentas
pOC10 Cloning vector derived from pOK12, replacement of the kan gene with the cat gene from pAV35, Cmr [19]
pTZ57R/t T/A Cloning vector, Apr Fermentas
pET30a Cloning and expression vector, Kanr Novagen
pBAD33 pBAD expression; pACYC184 ori; Cmr [31]
pCHL2 hp0405::PflaA kan , Kanr, Cmr This study
pCPY001 pUC18 containing minCHp, Apr This study
pCPY002 pCPY001 with cat inserted into the unique SphI site of minCHp, Apr, Cmr This study
pCPY003 pCHL2 containing minCHp under flaA promoter, hp0405::PflaA-minCHp kan , Kanr, Cmr This study
pCPY004 pET30a containing 6×his-minCHp , Kanr This study
pCPY005 pET30a containing 6×his-minCEc , Kanr This study
pCPY006 pCHL2 containing minCEc under flaA promoter, hp0405::PflaA-minCEc kan , Kanr, Cmr This study
pCPY007 pET30a containing 6×his-ftsZ , Kanr This study
pCPY008 pET30a containing 6×his-minD , Kanr This study
pCPY009 pBAD33 containing minCHp, Cmr This study
pCPY010 pBAD33 containing minCEc, Cmr This study
pOC0405 pOC10 containing hp0405, Cmr This study
pTZ-PflaA pTZ57R/t containing H. pylori flagella promoter, Apr This study
pTZ-PflaAKm pTZ-PflaA containing kan from pJMK30, Apr, Kanr This study