Table 1. Strains and plasmids used in this study.

Strain or plasmid	Genotype or description	Source
Strains
E.coli
Top10	F-mcrΔ (mrr-hsdRMS-mcrBC) 80lacZΔM15 lacXΔ74 recA1 deoR araD139Δ (ara-leu) 7697 galU galKrpsL (StrR) endA1 nupG	Invitrogen
BL21(DE3)	F– dcm ompT hsdS (rB – mB –) gal λ(DE3)	Stratagene
MG1655	wild-type	[30]
H. pylori
NCTC 11637	wild-type, containing the entire minC	ATCC 43504
PY1	11637, minC::cat	This study
PY2	PY1, hp0405::PflaA-minCHp kan	This study
PY2-5	PY1, hp0405::PflaA-minCEc kan	This study
PY3	11637, hp0405::PflaA-minCHp kan	This study
PY3-1	11637, hp0405::PflaA-minCEc kan	This study
Plasmids
pAV35	chloramphenicol acetyltransferase (cat) cassette; Cmr	[18]
pJMK30	kanamycin resistance (kan) cassette; Kanr	[18]
pUC18	Cloning vector, Apr	Fermentas
pOC10	Cloning vector derived from pOK12, replacement of the kan gene with the cat gene from pAV35, Cmr	[19]
pTZ57R/t	T/A Cloning vector, Apr	Fermentas
pET30a	Cloning and expression vector, Kanr	Novagen
pBAD33	pBAD expression; pACYC184 ori; Cmr	[31]
pCHL2	hp0405::PflaA kan , Kanr, Cmr	This study
pCPY001	pUC18 containing minCHp, Apr	This study
pCPY002	pCPY001 with cat inserted into the unique SphI site of minCHp, Apr, Cmr	This study
pCPY003	pCHL2 containing minCHp under flaA promoter, hp0405::PflaA-minCHp kan , Kanr, Cmr	This study
pCPY004	pET30a containing 6×his-minCHp , Kanr	This study
pCPY005	pET30a containing 6×his-minCEc , Kanr	This study
pCPY006	pCHL2 containing minCEc under flaA promoter, hp0405::PflaA-minCEc kan , Kanr, Cmr	This study
pCPY007	pET30a containing 6×his-ftsZ , Kanr	This study
pCPY008	pET30a containing 6×his-minD , Kanr	This study
pCPY009	pBAD33 containing minCHp, Cmr	This study
pCPY010	pBAD33 containing minCEc, Cmr	This study
pOC0405	pOC10 containing hp0405, Cmr	This study
pTZ-PflaA	pTZ57R/t containing H. pylori flagella promoter, Apr	This study
pTZ-PflaAKm	pTZ-PflaA containing kan from pJMK30, Apr, Kanr	This study