Table 1. DNA substrates employed in the study.
| Oligo number | Name of oligomers | Sequence (5′→ 3′) | Length (nt) | Oligomer no. annealed for DS substrate | Name of DS substrate |
| Oligonucleotides for AP endonuclease activity | |||||
| 1 | subSS-19AP | *ggcgaacgagacgagggc(AP)gctggaaagg | 29 | ||
| 2 | subSS-19AP(T) | cctttccagcTgccctcgtctcgttcgcc | 29 | 1and 2 | subAP·T/subDS-AP |
| 3 | subSS-19AP(A) | cctttccagcAgccctcgtctcgttcgcc | 29 | 1and 3 | subAP·A |
| 4 | subSS-19AP(G) | cctttccagcGgccctcgtctcgttcgcc | 29 | 1and 4 | subAP·G |
| 5 | subSS-19AP(C) | cctttccagcCgccctcgtctcgttcgcc | 29 | 1and 5 | subAP·C |
| Oligonucleotides for 3′→5′ exonuclease activity | |||||
| 6 | subSS-Exo | *gggactctcgaggaatgcg | 19 | ||
| 7 | subSS-Rec | ggcgcattcctcgagagtccc | 21 | 6 and 7 | subDS-REC |
| 8 | subSS-Blunt | cgcattcctcgagagtccc | 19 | 6 and 8 | subDS-BLUNT |
| 9 | subSS-Nick1 | cagctaatggctagcggc | 18 | ||
| 10 | subSS-Nick2 | gccgctagccattagctgcgcattcctcgagagtccc | 37 | 6, 9 and 10 | subDS-NICK |
SS is single-strand; DS is double-strand; nt is nucleotides; AP is the abasic site; asteriks (*) denotes the γ-32P radioactively labelled terminus.