Table 3. Reference genes primers for qPCR.
Gene name | Accession number | Primer sequence | Forward/ Reverse | Annealing T(°C) | Size (bp) |
HPRT | AY283372 | AGCTTGCTGGTGAAAAGGAC | F | 56 | 104 |
TTATAGTCAAGGGCATATCC | R | ||||
RPS19 | XM_533657 | CCTTCCTCAAAAAGTCTGGG | F | 61 | 95 |
GTTCTCATCGTAGGGAGCAAG | R | ||||
RPL8 | XM_532360 | CCATGAATCCTGTGGAGC | F | 55 | 64 |
GTAGAGGGTTTGCCGATG | R | ||||
SRPR | XM_546411 | GCTTCAGGATCTGGACTGC | F | 61 | 81 |
GTTCCCTTGGTAGCACTGG | R | ||||
RPL13 | AJ388525 | GCCGGAAGGTTGTAGTCGT | F | 61 | 87 |
GGAGGAAGGCCAGGTAATTC | R | ||||
GUSB | NM_001003191 | AGACGCTTCCAAGTACCCC | F | 62 | 103 |
AGGTGTGGTGTAGAGGAGCAC | R | ||||
GAPDH | NM_001003142 | TGTCCCCACCCCCAATGTATC | F | 58 | 100 |
CTCCGATGCCTGCTTCACTACCTT | R | ||||
B2MG | XM_535458 | TCCTCATCCTCCTCGCT | F | 61 | 85 |
TTCTCTGCTGGGTGTCG | R | ||||
RPS5 | XM_533568 | TCACTGGTGAGAACCCCCT | F | 62.5 | 141 |
CCTGATTCACACGGCGTAG | R |
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-2-Microglobulin (B2MG), Ribosomal protein S5 (RPS5), Ribosomal protein S19 (RPS19), Hypoxanthine phosphoribosyltransferase (HPRT), Ribosomalprotein L8 (RPL8), B-Glucuronidase (GUSB), Signal recognition particle receptor (SRPR), and Ribosomal protein L13 (RPL13).