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HeLa cells were transfected with the indicated GFP-tagged SAC1 constructs (green), costained with anti-GM130 antibodies (red) or Sec61β (red) and analyzed by confocal immunofluorescence microscopy. (A) SAC1(478–549); (B) GFP-SAC1(478–543); (C) GFP-SAC1(501–549); (D) GFP-SAC1(512–549). Scale bar, 50 µm.
