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. 2013 Aug 1;8(8):e69879. doi: 10.1371/journal.pone.0069879

Figure 1. Schematic representation of plasmid DNA used for producing stable cell lines.

Figure 1

Plasmid pFBGR-bsd contains the GFP gene under the dual control of the cytomegalovirus IE promoter (CMV) and baculovirus p10 promoter (p10) flanked by AAV-2 inverted terminal repeats (ITR). bla, β-lactamase (ampicillicin-resistance gene). bsd, blasticidin-S deaminase gene. ColE1, bacterial origin of replication. (Lower) Linear illustration of pFBGR-bsd indicates the rescued forms of the ITR-flanked transgene. The linear, single-stranded AAV virion genome is represented by a solid thin line flanked by the inverted terminal repeats (ITRs, filled rectangles). The duplex CELiD-vector DNA is represented by the open rectangle flanked by AAV ITRs.