Figure 7. CELiD vector DNA expression in vitro.

(A) HEK 293 cells transiently transfected with CELiD-GFP DNA or plasmid pFBGR for green fluorescent protein (GFP) expression. Both DNAs harbor an identical gene expression cassette encoding GFP and were transfected using equivalent copy numbers. Images were taken at day 6 post-transfection. Magnification, 10x objective (with digitally-enlarged insert). (B) HEK 293 cells transiently transfected with equal amounts of CELiD-LacZnls DNA or plasmid pFB-CMV-LacZnls. Both DNAs harbor an identical gene expression cassette encoding β-galactosidase with a nuclear localization signal (LacZnls). X-gal staining of cells at three days post-transfection. Magnification, 10x objective (with digitally-enlarged insert).