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. 2013 Aug 1;8(8):e70001. doi: 10.1371/journal.pone.0070001

Figure 5. Mapping the cis element that mediates TRIMs inhibition of HBV Enh II activity.

Figure 5

(A) Schematic representation to map the cis element mediating TRIMs inhibition of HBV Enh II activity. Three overlapping DNA fragments covering the entire HBV Enh II region were placed in the up-stream of a firefly luciferase coding sequence to yield three reporter plasmids, pGL4.10-HBV Enh II-1, pGL4.10-HBV Enh II-2 and pGL4.10-HBV Enh II-3, respectively. (B to D) 0.2 µg of the indicated reporter plasmid was co-transfected into HepG2 cells in 24 well plates with 0.2 µg of a vector plasmid or a plasmid expressing the indicated TRIM proteins, with 0.1 µg of pCMV-renilla luciferase as an internal control. Two days post transfection, cells were harvested and the luciferase activity was analyzed with a dual-luciferase kit. The mean and standard deviations (n = 4) were presented. * and ** indicate P<0.05 and 0.01, respectively.