Figure 6. RING finger and PRYSPRY domains of TRIM41 are essential for the inhibition of HBV Enh II activity.

0.4 µg of plasmids expressing wild type or mutant TRIM41 were transfected into HepG2 in 24 well plates. The cells were harvested at two days post transfection. Expression of TRIM41 was determined by Western blot assays (A) or immunofluorescence (B). (C) 0.2 µg of pGL4.10-HBV Enh II was co-transfected into HepG2 cells in 24 well plates with 0.2 µg of a vector plasmid or plasmid expressing the wild type or indicated mutant TRIM41, with 0.1 µg of pCMV-renilla luciferase as an internal control. Two days post transfection, cells were harvested and the luciferase activity was analyzed with a dual-luciferase kit. The mean and standard deviations (n = 4) were presented. * and ** indicate P<0.05 and 0.01, respectively.