Figure 2. In vitro specificity test of ⁶⁸Ga-NODAGA-Z_HER2:S1 (A), ⁶⁸Ga-NOTA-Z_HER2:S1 (B) and ⁶⁸Ga-DOTA-Z_HER2:S1 (C) binding to HER2 expressing SKOV-3 cells.

Dishes containing cells were incubated with 27 pM radiolabeled conjugated. One group of dishes was pre-saturated with 1000-fold excesses of non-labeled Z_HER2:S1 to block the binding site of HER2. Cell-associated radioactivity was calculated as percentage of total added radioactivity. Data are presented as mean values for three cell dishes and standard deviations.