Table 2. Labeling yield (decay corrected), radiochemical purity and stability of 68Ga-labeled Affibody molecules.
Conjugate | Yield | Purity | Stability under EDTA challenge* (EDTA) | Stability under EDTA challenge* (control) |
68Ga-DOTA-ZHER2:S1 | 94.6±1.0% | 99±2.1% | 99.5±0.1% | 99.2±0.1% |
68Ga-NOTA-ZHER2:S1 | 87.4±10.4% | 98.3±2.1% | 99.7±0.0% | 99.5±0.1% |
68Ga-NODAGA-ZHER2:S1 | 94.6±4.7% | 99.3±0.2% | 99.5±0.1% | 99.4±0.1% |
To evaluate stability, conjugates were incubated with 500-fold excess of EDTA for 1 h and then analyzed using ITLC. Control samples were incubated in PBS. The experiments were performed in duplicates. The data present an average conjugate-associated radioactivity and maximum error.