Table 1. QRT-PCR primers and probes used in this study.
| Gene | Type | Sequence (5′–3′) |
| Grin1(NMDAR NR1) | Forward | GTC CAT CTA CTC TGA CAA GAG |
| Reverse | AAA CCA GAC GCT GGA CTG GT | |
| Probe | f TCC ACC TGA GCT TCC TTC GCA CCGq | |
| Grin2a(NMDAR NR2A) | Forward | ACC TCG CTC TGC TCC AGT TT |
| Reverse | GTT GTG GCA GAT GCC CGT AA | |
| Probe | f CAG TGT CTC CAG CTC TTC CAT CTC ACq | |
| Grin2b(NMDAR NR2B) | Forward | TGG TCT TCT CCA TCA GCA GA |
| Reverse | GTT CAT CAC GGA TTG GCG CT | |
| Probe | f ATC TAC AGC TGT ATC CAC GGA GTA GCq | |
| CCL2 | Forward | CTG GAG CAT CCA CGT GTT G |
| Reverse | TGG GAT CAT CTT GCT GGT GA | |
| Probe | f AGC CAG ATG CAG TTA ACG CCC CAC T q | |
| AIF-1 (Iba1) | Forward | GCA ATT CCT CGA TGA TCC CA |
| Reverse | ATG TAC TTC ACC TTG AAG GCT | |
| Probe | f CAG CAA TGA TGA GGA TCT GCC GTC CAq | |
| GFAP | Forward | CTC AAG AGG AAC ATC GTG GT |
| Reverse | TGC TCC TGC TTC GAG TCC TT | |
| Probe | f TGA CCT CAC CAT CCC GCA TCT CCAq | |
| 18S | Forward | AGA AAC GGC TAC CAC ATC CA |
| Reverse | CTC GAA AGA GTC CTG TAT TGT | |
| Probe | tAGG CAG CAG GCG CGC AAA TTA Cq |
Primers and probes used for the quantification of mRNA from NMDA receptor subtypes NR1, NR2A and NR2B (Grin); microglia marker Iba-1 (AIF-1), astrocyte (GFAP) and CCL2; f, Reporter dye1 (FAM:6-carboxyfluorescein); t, Reporter dye2 (TET:Tetrachloro-6-carboxyfluorescein); q, Quencher dye (TAMRA: 6-carboxytetramethyl1-rhodamine).