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. 2013 Aug 1;8(8):e70622. doi: 10.1371/journal.pone.0070622

Figure 4. Spi1 mediates monocyte-specific IL1B expression.

Figure 4

(A) Spi1 ChIP for IL1B and TNF in control and LPS-treated THP-1 cells. (B) Transcription factor mRNA expression profiles in HEK293 and THP-1 cells. A third panel (and data in Figure S5H) displays ectopic mRNA expression of Spi1 in transfected HEK293. (C) IL1BXT-Luc reporter activity for ectopic expression of indicated factors in HEK293. (D) Endogenous IL1B mRNA expression in transfected HEK293. (E) ChIP for endogenous TBP, Pol II and H3 with ectopic Spi1 in HEK293. Vertical gray bars designating important gene landmarks are as described in Figure 3.