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. 2013 Aug 1;8(8):e70622. doi: 10.1371/journal.pone.0070622

Figure 5. LPS-activated C/EBPβ interaction with Spi1 differentiates induction of IL1B and TNF.

Figure 5

(A) NF-κB and C/EBPβ ChIP for THP-1 cells, as indicated. (B) Effect of ectopic expression of IκBα super repressor (IκBαSR) on IL1B XT-Luc reporter activity in HEK293 cotransfected with indicated factors. (C) Effect of dnC/EBPβ titration on IL1B XT-Luc reporter activity in HEK293. (D) Effect of C/EBPβ and NF-κB binding site mutations on IL1BXT-Luc reporter activity in RAW264.7. (E) XT-Luc reporter activity, as indicated in HEK293 cotransfected with C/EBPβ, NF-κB and Spi1 siRNA. (F) P-TEFb and (G) BRD4 ChIP in THP treated, as indicated.