Figure 2. Effects of divalent cation on protein kinase activity.

A) Representative autoradiograph scan showing relative intensity of phosphorylation in the presence of Mn²⁺ and Mg²⁺, Mn²⁺ alone, and Mg²⁺ alone. Graphs summarise the results of N = 5 individual experiments for Manganese (black bars) and Magnesium (white bars). B) Graph showing the abilities of different isoforms to phosphorylate βic, relative to p110α, N≥3 experiments. C) Graphs comparing the degree of autophosphorylation for the two oncogenic mutants, H1047R (black bars) and E545K (white bars) to p110α (hatched bars), N≥3 experiments.