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. 2013 Aug 1;8(8):e70098. doi: 10.1371/journal.pone.0070098

Figure 4. Tryptophan is a specific marker of diverse immature Coffea arabica green beans.

Figure 4

(A) The total ion chromatograms derived from the LC-MS analyses of either the catimore 5175-1 immature or mature bean extract. The red arrowhead indicates a characteristic peak that specifically appeared in the green bean sample. (B) The extracted ion chromatogram (XIC) of 205.0941 (+) retained at 5.00–5.40 minutes and the mass spectrum are shown. (C) Box-and-whisker plots derived from the tryptophan in diverse varieties. The mass intensities (Y-axis) were normalized to the sum total ion counts obtained from each sample. The developmental stages (X-axis) were abbreviated as I (immature), SM (semi-mature), M (mature) and OR (overripe).