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. 2013 Aug 1;8(8):e70511. doi: 10.1371/journal.pone.0070511

Figure 6. Promotions of cell proliferation and invasion by Fibulin-3 siRNA in QGY-7703 cells.

Figure 6

A. Fibulin-3 siRNA noticeably downregulated expression of Fibulin-3 protein. Scramble and Fibulin-3 siRNA were transfected into QGY-7703 cells for 24 h. Relative Fibulin-3 expressions were detected by western blot. B. Fibulin-3 siRNA significantly increased cell viability in HCC cells. Cells transfected with scramble siRNA (ss) and Fibulin-3 siRNA (#1 and #2) were seeded into 96-well plates and cultured for 24 h. The cell viabilities were determined using MTT assays. The data are means ± SD of three independent experiments, *P<0.05. C. Fibulin-3 siRNA promoted cell invasion in vitro. QGY-7703 cells were seeded into transwell chambers coated with Matrigel. After 24 h, invaded cells stained by 0.1% crystal violet were counted. The data are means ± SD of three independent experiments, *P<0.05.