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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Nitric Oxide. 2012 Jul 20;27(4):201–209. doi: 10.1016/j.niox.2012.07.002

Figure 3. Inhibition of proinflammatory gene expression of iNOS and IL-1β by High dose of copper is NF-κB independent.

Figure 3

A: Cu(I) does not impair NF-κB activity in LPS-treated BV2 cells. BV2 microglia were challenged with 1 or 100 μM Cu(I) and 8 hrs later stimulated with 0, or 1000ng/mL LPS. NF-κB activity was measured 3 hrs later. The data shown are representative of three independent experiments. B: Three hrs after LPS treatment, total RNA was isolated and subjected to real-time RT/PCR. The data were calculated and normalized by 2-ΔΔCt Method and induction of NOS2 and IL1β mRNA was expressed as ratios over β-Actin. The data shown are representative of three independent experiments. * LPS 10 vs LPS 10 + Cu(I) 100; + LPS 1000 vs LPS 1000 + Cu(I) 100; # LPS 1000 + Cu(I) vs. LPS 1000 + Cu(I) 100 indicate p < 0.05.