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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Chem Res Toxicol. 2013 Apr 4;26(4):622–628. doi: 10.1021/tx4000667

Figure 2.

Figure 2

GCG enhances DNA cleavage mediated by human topoisomerase IIα (hTIIα; left) and IIβ(hTIIβ; right) with an activity that is similar to that of its epimer, EGCG. DNA cleavage reactions were carried out in the presence of 0–300 μM EGCG (open circles) or GCG (closed circles). Levels of DNA cleavage were quantified from the band of linear DNA and expressed relative to reactions carried out in the absence of catechin. Error bars represent standard deviations for three or four independent experiments. Representative agarose gels of DNA cleavage reactions carried out in the presence GCG are shown at the top for the respective topoisomerase II isoform. FI represents negatively supercoiled circular DNA, FII represents nicked DNA, and FIII represents linear DNA.