Figure 8.
GCG does not enhance DNA cleavage mediated by human topoisomerase IIα (hTIIα; left) and IIβ(hTIIβ; right) by adducting DNA. DNA was incubated in the absence (open bars) or presence (closed bars) of 200 μM GCG for 5 min, centrifuged through a spin column to remove the bioflavonoid, and then used in DNA cleavage reactions. DNA cleavage was carried out in the absence (No Drug) or presence (GCG) of 200 μM GCG. Error bars represent standard deviations for three or four independent experiments.