Skip to main content
. Author manuscript; available in PMC: 2014 Oct 30.
Published in final edited form as: J Proteomics. 2013 Mar 14;92:10.1016/j.jprot.2013.01.033. doi: 10.1016/j.jprot.2013.01.033

Figure 1. Experimental workflow.

Figure 1

1) Samples are collected. 2) Proteins are extracted from the sample, via chemical precipitation for body fluids and detergent extraction for cells. 3) Proteins are fractionated via SDS-PAGE. 4) Proteins are subjected to GeLC-MS/MS. 5) RAW files are converted to mascot generic files (mgf). 6) Database searching is performed using ProteinPilot. 7) ProteinPilot Descriptive Statistics Template (PDST) is used to tabulate PTMs. 8) SAS is used to determine the statistical significance of differentially identified PTM frequencies.