Table 4.
Statistical analysis of PTMs identified in both cohorts.
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| Modification | p-value | #NP | #CP | Median NP | Median CP | Modification | p-value | #NP | #CP | Median NP | Median CP |
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| Acetyl(N-term) | 0.020 | 9 | 9 | 12.49 | 10.09 | Formyl(N-term) | 0.345 | 9 | 9 | 1.68 | 1.42 |
| Protein Terminal Acetyl(N-term) | 0.058 | 7 | 9 | 5.64 | 29.93 | Gln->pyro-Glu(N-term) | 0.041 | 9 | 9 | 258.24 | 320.24 |
| Amidated(C-term) | 0.395 | 5 | 3 | 0.06 | 0.00 | Glu->pyro-Glu(N-term) | 0.862 | 9 | 9 | 27.78 | 31.25 |
| Amino(Y) | 0.660 | 2 | 3 | 0.00 | 0.00 | GlyGly(K) | 0.929 | 7 | 5 | 0.10 | 0.12 |
| Ammonia-loss(N) | 0.069 | 9 | 9 | 0.92 | 0.43 | GlyGly(S) | 0.470 | 5 | 5 | 0.04 | 0.06 |
| Arg-add(N-term) | 1.000 | 3 | 3 | 0.00 | 0.00 | Hex(K) | 0.146 | 4 | 6 | 0.00 | 0.11 |
| Arg-loss(C-term) | 0.443 | 6 | 4 | 0.21 | 0.00 | Hex(Y) | 1.000 | 1 | 1 | 0.00 | 0.00 |
| Carbamyl(K) | 0.861 | 9 | 5 | 0.12 | 0.14 | Lys-add(N-term) | 0.723 | 7 | 5 | 0.14 | 0.07 |
| Carbamyl(N-term) | 0.035 | 9 | 9 | 4.20 | 3.34 | Lys-loss(C-term) | 0.290 | 5 | 6 | 0.11 | 0.14 |
| Carboxy(D) | 0.594 | 2 | 1 | 0.00 | 0.00 | Met->Hcy(M) | 1.000 | 1 | 1 | 0.00 | 0.00 |
| Carboxy(E) | 0.384 | 4 | 2 | 0.00 | 0.00 | Methyl(E) | 0.130 | 9 | 9 | 1.41 | 1.11 |
| Cation:K(D) | 0.041 | 9 | 9 | 1.79 | 1.34 | Methyl(H) | 0.445 | 3 | 2 | 0.00 | 0.00 |
| Cation:K(E) | 0.728 | 9 | 9 | 1.29 | 1.60 | Methyl(I) | 0.739 | 4 | 4 | 0.00 | 0.00 |
| Cation:Na(D) | 0.041 | 9 | 9 | 0.35 | 0.25 | Methyl(K) | 0.303 | 7 | 8 | 0.11 | 0.18 |
| Cation:Na(E) | 0.176 | 9 | 9 | 0.79 | 0.75 | Methyl(L) | 0.224 | 5 | 3 | 0.05 | 0.00 |
| Deamidated(N) | 0.069 | 9 | 9 | 214.09 | 204.22 | Methyl(Q) | 0.952 | 2 | 2 | 0.00 | 0.00 |
| Deamidated(Q) | 0.029 | 9 | 9 | 131.38 | 125.10 | Methyl(R) | 0.408 | 1 | 3 | 0.00 | 0.00 |
| Deamidated(R) | 0.489 | 9 | 9 | 35.81 | 34.63 | Nitro(W) | 0.034 | 3 | 7 | 0.00 | 0.41 |
| Dehydrated(D) | 0.603 | 9 | 7 | 0.21 | 0.11 | Nitro(Y) | 0.664 | 9 | 8 | 0.22 | 0.24 |
| Dehydrated(E) | 0.035 | 9 | 9 | 0.31 | 0.38 | Oxidation(D) | 0.489 | 9 | 9 | 1.79 | 1.80 |
| Dehydrated(S) | 0.232 | 8 | 7 | 0.14 | 0.06 | Oxidation(F) | 0.545 | 9 | 9 | 0.83 | 0.58 |
| Dehydrated(T) | 0.203 | 9 | 8 | 0.21 | 0.11 | Oxidation(H) | 0.152 | 9 | 9 | 17.28 | 19.76 |
| Dehydrated(Y) | 1.000 | 1 | 1 | 0.00 | 0.00 | Oxidation(K) | 0.233 | 9 | 9 | 0.81 | 1.08 |
| Delta:H(2)C(2)(H) | 0.664 | 9 | 9 | 10.21 | 9.40 | Oxidation(M) | 0.006 | 9 | 9 | 551.35 | 623.48 |
| Delta:H(2)C(2)(K) | 0.304 | 9 | 9 | 0.83 | 0.84 | Oxidation(N) | 0.728 | 9 | 9 | 1.75 | 1.82 |
| Delta:H(2)C(2)(N-term) | 0.081 | 9 | 9 | 10.74 | 8.64 | Oxidation(P) | 0.049 | 9 | 9 | 11.41 | 12.84 |
| Delta:H(4)C(2)(H) | 1.000 | 2 | 2 | 0.00 | 0.00 | Oxidation(R) | 0.931 | 9 | 9 | 1.57 | 1.43 |
| Delta:H(4)C(2)(K) | 0.506 | 2 | 1 | 0.00 | 0.00 | Oxidation(W) | 0.176 | 9 | 9 | 29.46 | 27.05 |
| Delta:H(4)C(2)(N-term) | 1.000 | 9 | 8 | 0.30 | 0.28 | Oxidation(Y) | 0.120 | 9 | 9 | 5.03 | 4.53 |
| Deoxy(D) | 0.506 | 2 | 1 | 0.00 | 0.00 | Phospho(S) | 0.408 | 3 | 1 | 0.00 | 0.00 |
| Dethiomethyl(M) | 0.703 | 5 | 3 | 0.34 | 0.00 | pyro-Glu(P) | 0.095 | 9 | 9 | 0.35 | 0.22 |
| Dioxidation(F) | 0.716 | 5 | 5 | 0.10 | 0.09 | Propionamide(C) | 1.000 | 9 | 9 | 1000.00 | 1000.00 |
| Dioxidation(K) | 0.664 | 7 | 8 | 0.21 | 0.12 | Propionamide(K) | 0.489 | 9 | 9 | 1.03 | 0.59 |
| Dioxidation(M) | 0.304 | 9 | 9 | 13.11 | 17.11 | Propionamide(N-term) | 1.000 | 9 | 9 | 2.06 | 2.09 |
| Dioxidation(P) | 0.788 | 6 | 5 | 0.11 | 0.11 | PyruvicAcidIminyl(K) | 0.506 | 2 | 1 | 0.00 | 0.00 |
| Dioxidation(R) | 0.693 | 4 | 0.00 | 0.00 | Trioxidation(W) | 0.058 | 7 | 3 | 0.61 | 0.00 | |
| Dioxidation(W) | 0.203 | 9 | 9 | 22.09 | 18.55 | Hydroxykynurenin(W) | 0.081 | 9 | 8 | 1.43 | 0.64 |
| Dioxidation(Y) | 0.103 | 8 | 8 | 0.22 | 0.71 | Kynurenin(W) | 0.069 | 9 | 9 | 5.85 | 4.48 |
| Formyl(K) | 0.198 | 4 | 1 | 0.00 | 0.00 | ||||||
NP, no pancreatitis (controls); #NP, number of NP samples in which a specific PTM has been identified; CP, no pancreatitis (controls); #CP, number of CP samples in which a specific PTM has been identified; Median, median frequency of PTM (per 1,000 potential cites); Bold = statistically significant p-value<0.005.