Table 1.
Sequences of Primers and Real-time PCR Conditions
| Gene | Primers (F=forward; R=reverse) | Amplicon size (bp) |
Annealing temperature (°C) |
|---|---|---|---|
| IL-6 | F: 5’-TTCCATCCAGTTGCCTTCTTG-3’ | 101 | 58 |
| R: 5’-TTGGGAGTGGTATCCTCTGTGA-3’ | |||
| COX-2 | F: 5’-TGAGTACCGCAAACGCTTCTC-3’ | 151 | 58 |
| R: 5’-TGGACGAGGTTTTTCCACCAG-3’ | |||
| SOD2 | F: 5’-ATGTTACAACTCAGGTCGCTCTTC-3’ | 135 | 56 |
| R: 5’-TGATAGCCTCCAGCAACTCTCC-3’ | |||
| catalase | F: 5’-GAACGAGGAGGAGAGGAAAC-3’ | 95 | 56 |
| R: 5’-TGAAATTCTTGACCGCTTTC-3’ | |||
| 18s | F: 5’-CGGCGACGACCCATTCGAAC-3’ | 99 | 58 |
| R: 5’-GAATCGAACCCTGATTCCCCGTC-3’ |