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. 2013 Jul 15;27(14):1610–1623. doi: 10.1101/gad.214080.113

Figure 1.

Figure 1.

Acute ATR inhibition causes rapid cell lethality and an inability to complete DNA replication after a replication stress challenge. (A–D) U2OS cells were treated with DMSO, 5 μM ATRi, and/or 3 mM HU for the indicated times and released into fresh growth medium for 10–14 d. Colonies were visualized by methylene blue staining. Results shown are mean ± standard error of the mean (SEM) of at least two independent experiments. (E,F) RPE-hTERT cells were labeled with 20 μM BrdU for 20 min, treated with 3 mM HU for 5 h (E) or 16 h (F) in the presence or absence of 5 μM ATRi, and then released into fresh growth medium containing 1 μg/mL nocodazole for 24 h prior to harvesting. Cells were then fixed, acid-denatured, stained with BrdU antibodies and propidium iodide, and analyzed by flow cytometry. Plots were made using Cyflogic software.