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. 2013 Jul 15;27(14):1610–1623. doi: 10.1101/gad.214080.113

Figure 2.

Figure 2.

ATR regulates DNA replication initiation and elongation. (A,B) RPE-hTERT cells were labeled with IdU for 20 min and then with CldU for 20 min in the presence of DMSO (red bars) or 5 μM ATRi (blue bars) during both labeling periods before harvesting for fiber labeling. (A) Representative replication tracks and quantification of the length of CldU (red) tracks in dual-labeled tracks are shown. (B) Origin initiation was scored as the percentage of red-only tracks. (C–F) RPE-hTERT cells were labeled with IdU for 20 min, treated with 2 mM HU for 2 h in the presence of DMSO (red bars) or 5 μM ATRi (blue bars), and then labeled with CldU for 20 min before harvesting for fiber staining. Representative images and quantification of CldU (C) and IdU (F) track lengths in dual-labeled fibers are shown. Percentage of collapsed forks (green-only tracks) (D) and newly initiated origins (red-only tracks) (E) were quantitated. In all experiments, data was collected from several experimental samples with high-quality DNA fibers. Error bars are SEM.