Fig. 4.

Representative proteomic data. (A) Representative 2-DE gel of CC group ovarian proteins is shown, with the spots identified in Table 3 denoted by arrows and numbers. (B) Quantitation of spots 47 and 67, both identified as MVP. Representative zoom-boxes for each treatment group are shown, as well as box and whisker plots of normalised spot volumes. The horizontal lines in the boxes show the median values, with the limits of the boxes showing the 25% and 75% quantiles and the whiskers showing the 10% and 90% quantiles. Common superscripts between groups, for each protein spot, denote statistically significant differences at P < 0.05. (C) Representative, not-quantitative 2-DE Western blots for MVP showing the localisation of a single spot at the locus of spot #47. D. Representative immunohistochemistry showing that MVP expression increases dramatically between day 55 and 140 of gestation in the fetal sheep ovary and is oocyte-specific. The inset box shows IgG-ve staining control.